Association between insulin-like growth factor-I, thyroxine and follicle stimulating hormone on the survival and in vitro development of caprine preantral follicles
Palavras-chave:
Caprine, Hormone, in vitro culture, preantral follicles, ultrastructural analysisResumo
The aim of this study was to investigate the influence of the interaction between follicle stimulating hormone obtained from Human Pituitary (hFSH; 10 ng/mL), Insulin-like Growth Factor Type 1 (IGF-I; 100 ng/mL) and thyroxine (T4; 10, 20 ng/mL) on survival, activation and growth of preantral follicles (PAF) cultured in vitro. Fragments of ovarian cortex collected from 17 nonpregnant adult goats underwent different treatments that included α-MEM+ medium, IGF-I, hFSH, T4 and were processed for classic histology and transmission electron microscopy. The results showed a reduction in the percentage of normal follicles, after one and seven days of culture in media α-MEM+ (cultured control), T4(10)/IGF-I, T4(20)/IGF-I/hFSH and in media α-MEM+, IGF-I/hFSH, T4(20)/IGF-I/hFSH, T4(20)/IGF-I/hFSH, when compared to the non-cultured control. However, the medium containing T4(20)/hFSH showed a follicular survival rate higher than the
