Susceptibility of dermatophytic fungi to commonly used disinfectants

Angelita Reis Gomes, Isabel Martins Madrid, Stefanie Bressan Waller, Alessandra Jacomelli Teles, Otávia Almeida Martins, Ângela Leitzke Cabana, Willian Silva Barros, Mário Carlos Araujo Meireles


This study aimed evaluate the antidermatophytic activity of three commercial disinfectants commonly used for environmental control of microorganisms in veterinary medicine. Sodium hypochlorite at 40 μL/mL, chloro-phenol derived at 30 μL/mL and chlorhexidine digluconate at 66.7 μL/mL were tested against 14 strains of dermatophytes, identified as Microsporum canis (n: 3) and Microsporum gypseum (n: 11). The tests was performed in accordance with guidelines of the Clinical and Laboratory Standards Institute (CLSI), documents M38-A2 and M51-A, adapted to disinfectants. In the microdilution broth test, chlorhexidine digluconate had MIC values (Minimum Inhibitory Concentration) of 4.16 μL/mL and MCF (Minimum Fungicidal Concentration) from 4.16 to 8.33 μL/mL, while chloro-phenol derived obtained MIC and MCF of 1.87 μL/mL, and both disinfectants had fungicidal activity at concentrations below the recommended. Sodium hypochlorite obtained MIC from 10 to 80 μL/mL and MFC of 40 to 80 μL/mL, requiring at most isolates twice the recommended concentration to achieve same activity. In the disc diffusion test, the mean inhibition zones for chlorhexidine digluconate was 10.53 mm, for chloro-phenol of 9.9 mm and for sodium hypochlorite was 6.2 mm.  Chlorhexidine digluconate and chloro-phenol presented a significant reduction in the growth of dermatophytes, while sodium hypochlorite in concentration recommended showed a low antifungal activity against tested isolates.


Antidermatophytic activity, disinfection, microdilution broth technique, disc diffusion

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Revista Brasileira de Ciência Veterinária - RBCV