Automated method and not automated: the evaluation of hematological parameters of rats

Auteurs-es

  • Julio Brando Messias Universidade de Pernambuco - UPE
  • Ivana Porto Farias Universidade de Pernambuco - UPE
  • Inalda Maria de Oliveira Messias Unirversidade de Pernambuco - Campus Petrolina - UPE
  • Ana Luiza Germano Ramos
  • Mércia Cristina de Magalhães Caraciolo Fundação Oswaldo Cruz - Centro de Pesquisa Aggeu Magalhães (FioCruz/CPqAM)

Mots-clés :

utomation, hematology, blood cell count, blood

Résumé

The aim of this study was to verify blood compatibility between automated and non-automated methodology in rats. Counts were made of haematological variables 10 Wistar rat, Rattus norvegicus. For the automated method were used the ABX-Pentra-60c + equipment and Celldyn-3200. For non-automated method the hemocytometer was used, the counts of erythrocytes, leukocytes and platelets using the liquid Hayem (1: 200), the liquid Türker (1:20) and the liquid Brecher and Conqrite (1: 200), respectively. The determination of the hematocrit was obtained using a microhematocrit centrifuge, hemoglobin concentration by cyanmethaemoglobin method (Drabkin liquid) and absolute RBC indices, calculated by the results of red series. The differential leukocyte count was performed on blood extensions (fast Panotic). Data were analyzed by analysis of variance - ANOVA followed by Tukey test at 5% significance level. The values obtained by hemocytometer compared to hematological counters showed significant differences (p < 0.05) for neutrophils, eosinophils and basophils. The results showed that the counts made with the devices used were reliable and fast, but are not indicated for the differential count of white blood cell.

Téléchargements

Les données sur le téléchargement ne sont pas encore disponible.

Bibliographies de l'auteur-e

Julio Brando Messias, Universidade de Pernambuco - UPE

Professor de Histologia e Embriologia do Instituto de Ciências Biológicas da Universidade de Pernambuco (ICB/UPE)

Especialista em Morfologia (UFPE), Especialista em Metodologia do Ensino (UPE), Mestre em Ciência Veterinária (UFRPE) e Doutor em Ciências Farmacêuticas (UFPE)

Ivana Porto Farias, Universidade de Pernambuco - UPE

Técnica do Laboratório de técnicas Histológicas do Instituto de Ciências Biológicas - ICB/UPE

Inalda Maria de Oliveira Messias, Unirversidade de Pernambuco - Campus Petrolina - UPE

Professora de Semiologia e Semiotécnica, Clínica Médica e Clínica Cirúrgica, Exames Laboratoriais e Biosseegurança para Enfermagem.

Mestranda em Engenharia Biomédica - UPE

Ana Luiza Germano Ramos

Técnica de Patologia Clínica do Hospital PROCAPE-UPE

Mércia Cristina de Magalhães Caraciolo, Fundação Oswaldo Cruz - Centro de Pesquisa Aggeu Magalhães (FioCruz/CPqAM)

Pesquisadora do Centro de Pesquisa Aggeu Magalhães (FioCruz)

Automated method and not automated: the evaluation of hematological parameters of rats

Téléchargements

Publié-e

2017-10-17

Numéro

Rubrique

Patologia e Análises Clínica Veterinária