Automated method and not automated: the evaluation of hematological parameters of rats

Abstract

The aim of this study was to verify blood compatibility between automated and non-automated methodology in rats. Counts were made of haematological variables 10 Wistar rat, Rattus norvegicus. For the automated method were used the ABX-Pentra-60c + equipment and Celldyn-3200. For non-automated method the hemocytometer was used, the counts of erythrocytes, leukocytes and platelets using the liquid Hayem (1: 200), the liquid Türker (1:20) and the liquid Brecher and Conqrite (1: 200), respectively. The determination of the hematocrit was obtained using a microhematocrit centrifuge, hemoglobin concentration by cyanmethaemoglobin method (Drabkin liquid) and absolute RBC indices, calculated by the results of red series. The differential leukocyte count was performed on blood extensions (fast Panotic). Data were analyzed by analysis of variance - ANOVA followed by Tukey test at 5% significance level. The values obtained by hemocytometer compared to hematological counters showed significant differences (p < 0.05) for neutrophils, eosinophils and basophils. The results showed that the counts made with the devices used were reliable and fast, but are not indicated for the differential count of white blood cell.